New PDF release: Advances in Applied Microbiology, Vol. 3

By Wayne W Umbreit

ISBN-10: 0120026031

ISBN-13: 9780120026036

Meant for researchers in utilized microbiology and environmental engineers, this e-book covers such issues as environmental overview of biotechnological methods and microbial alterations of haloaromatic and haloaliphatic compounds.

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Substances that protect against slow freezing are ineffective during rapid freezing, and conversely, substances that protect cells on rapid freezing are ineffective when the cell suspensions are frozen slowly. F. METHODOF DRYING 1. From the Frozen State Some authors state that a specific temperature during drying or a rate of dehydration is optimal for the survival of bacteria; others maintain the opposite view. Actually there are few studies in which the rate of drying was not obscured by the fact that, to achieve the different drying rates, the temperature of the sample was changed.

Some unpublished data (Heckly, 1953) indicated that conditions obtained by drying at -18OC. also resulted in a higher percentage survival of P. pestis when tested immediately after drying than when the preparations were dried at -3OOC. 2. %-package” Desiccant The inclusion of a package of desiccant in the same container as dried yeast was the subject of a patent issued to Ohlhaver in 1912 (Mitchell 42 ROBERT J. HECKLY and Enright, 1957). The use of an “in-package” desiccant (calcium oxide) has been further explored by Mitchell and Enright ( 1957)In a method described by Barratt and Tatum (1950) phosphorus pentoxide was placed in the bottom of a large tube and then covered with cotton.

Considerable machine shop facilities were required to build some of the equipment, particularly the larger units, described by Flosdorf and Mudd ( 1935). The apparatus described by Strumia et al. (1941) was a modification of Flosdorf‘s metal condenser system. The Strumia apparatus, which was able to process bottles up to 20-liter capacity, was designed primarily for processing serum and plasma and seems not particularly well suited to the lyophilization of bacterial cultures. Wyckoff and Lagsdin (1944) described “a simple outfit for drying plasma from the frozen state” which had several useful features, some of which are evident in equipment now commercially available.

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Advances in Applied Microbiology, Vol. 3 by Wayne W Umbreit


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