By P. D. Evans
Insect body structure is at present present process a revolution with the elevated software of molecular organic ideas to enquire the molecular mechanisms underlying the physiological responses to insect cells. Advances in Insect body structure has instituted a dedication to the e-book of top quality reports on molecular biology and molecular genetics in components the place they supply an elevated figuring out of physiological procedures in bugs. quantity 25 includes elevated insurance at the molecular biology of insect body structure.
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Additional resources for Advances in Insect Physiology, Vol. 25
There are several advantages in the use of baculoviruses instead of other eukaryotic expression systems: the expression vectors do not require a helper virus system; the viruses are non-pathogenic to vertebrates and plants; and no oncogenic or transforming elements are employed. It has also been demonstrated that insect cells will perform a wide range of post-translational processing events that are often required for biological activity of a recombinant protein. One other advantage which has not been well documented is the application of the baculovirus expression vector system to the study of insect ADVANCES I N INSECT VIROLOGY 23 proteins.
The viral replication cycle resembles that of other poxviruses, except for the appearance of occluded virus late in the infection. Virus-induced rounding of EAA-BTI cells is one of the early features of AmEPV infection, and suggests that virus infection results in a reorganization of the host cell cytoskeleton. In a recent study using immunofluorescent staining of the tubulin and f-actin components of the cytoskeleton, Marlow et al. (1992) demonstrated that the microtubules begin to depofymerize between 12 and 24 hpi, and by 48-72 hpi have further contracted to form a reduced network around the main cell body.
This virus was designated BacPAK6 (Kitts and Possee, 1993). Digestion of the virus DNA with Bsu36I removes part of ORF 603, the polyhedrin gene promoter, the P-galactosidase coding region and part of ORF 1629. When the Bsu361digested BacPAK6 virus DNA was used to transfect insect cells, very low recoveries of infectious virus were attained. Recircularization of linear BacPAK6 DNA produces a crippled virus which is unable to produce infectious virus in insect cells. If the same DNA was co-transfected with a plasmid transfer vector, however, virus yields were enhanced considerably with nearly 100% recovery of recombinant virus.
Advances in Insect Physiology, Vol. 25 by P. D. Evans