By G.D. Aurbach and Donald B. McCormick (Eds.)
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Numerous ceramic components have already confirmed their suitability for serial program in vehicle engines in very amazing methods, specifically in Japan, america and in Germany. despite the fact that, there's nonetheless a scarcity of reasonably priced caliber coverage strategies. lately, a brand new iteration of ceramic parts, for the use in power, transportation and surroundings platforms, has been built.
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Bautista et al. (1991) purified a 29-kDa IGFBP from human bone whose NH,terminal amino acid sequence agreed with that deduced from the nucleotide sequence of human IGFBP-5 at 9 of 15 positions (see below). Camacho-Hubner et al. (1992) purified 31-kDa IGFBP-5 and a 23-kDa immunoreactive fragment from T98G human glioblastoma cells that were identical to the first 30 and 9 NH,-terminal amino acid residues, respectively, deduced from the nucleotide sequence of IGFBP-5. Binoux et al. (1991a) purified a 22-kDa IGFBP-5 fragment from human CSF.
1991b) purified COOH-terminal fragments of rat IGFBP-3 by gel filtration followed by IGF-I1 affinity chromatography, suggesting that the fragments retained the ability to bind IGF-11. The binding affinities of these IGFBP fragments have not been determined. , 1991). , 1991b). , 1991a). INSULIN-LIKE GROWTH FACTORS 29 Deletion and site mutagenesis of IGFBP-1 and IGFBP-3 have been used to identify the IGF-binding site. Interpretation of these results is difficult because of the difficulty in distinguishing effects on protein conformation from direct effects on ligand binding.
Liver and Other Tissues. , 1988). , 1990). , 198913). b. Rat. , 1990). Using 15 pg total RNA, hybridization was strong in stomach and lung; weaker in kidney, intestine, muscle, heart, and skin; and not detectable in brain. , 1990). , 1991). , 1991). Takenaka et al. (1991) obtained similar results in 160-g rats using 50 pg total RNA: highest in liver; detectable in kidney; and undetectable in spleen, intestine, testes, brain, stomach, heart, and lung. , 1991). , 1992). 2. IGFBP-2 a. Rat. 5-kb IGFBP-2 mRNA was abundant in liver, stomach, brain, kidney, and 38 MATTHEW M.
Advances in Research and Applications by G.D. Aurbach and Donald B. McCormick (Eds.)