Download e-book for kindle: Advances in Research and Applications by G.D. Aurbach and Donald B. McCormick (Eds.)

By G.D. Aurbach and Donald B. McCormick (Eds.)

ISBN-10: 0080866441

ISBN-13: 9780080866444

ISBN-10: 012709847X

ISBN-13: 9780127098470

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Bautista et al. (1991) purified a 29-kDa IGFBP from human bone whose NH,terminal amino acid sequence agreed with that deduced from the nucleotide sequence of human IGFBP-5 at 9 of 15 positions (see below). Camacho-Hubner et al. (1992) purified 31-kDa IGFBP-5 and a 23-kDa immunoreactive fragment from T98G human glioblastoma cells that were identical to the first 30 and 9 NH,-terminal amino acid residues, respectively, deduced from the nucleotide sequence of IGFBP-5. Binoux et al. (1991a) purified a 22-kDa IGFBP-5 fragment from human CSF.

1991b) purified COOH-terminal fragments of rat IGFBP-3 by gel filtration followed by IGF-I1 affinity chromatography, suggesting that the fragments retained the ability to bind IGF-11. The binding affinities of these IGFBP fragments have not been determined. , 1991). , 1991b). , 1991a). INSULIN-LIKE GROWTH FACTORS 29 Deletion and site mutagenesis of IGFBP-1 and IGFBP-3 have been used to identify the IGF-binding site. Interpretation of these results is difficult because of the difficulty in distinguishing effects on protein conformation from direct effects on ligand binding.

Liver and Other Tissues. , 1988). , 1990). , 198913). b. Rat. , 1990). Using 15 pg total RNA, hybridization was strong in stomach and lung; weaker in kidney, intestine, muscle, heart, and skin; and not detectable in brain. , 1990). , 1991). , 1991). Takenaka et al. (1991) obtained similar results in 160-g rats using 50 pg total RNA: highest in liver; detectable in kidney; and undetectable in spleen, intestine, testes, brain, stomach, heart, and lung. , 1991). , 1992). 2. IGFBP-2 a. Rat. 5-kb IGFBP-2 mRNA was abundant in liver, stomach, brain, kidney, and 38 MATTHEW M.

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Advances in Research and Applications by G.D. Aurbach and Donald B. McCormick (Eds.)


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